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| Di Ciero,Luciana; Bellato,Cláudia de M.; Meinhardt,Lyndel W.; Ferrari,Fernanda; Castellari,Rafael R.; Marangoni,Sérgio; Novello,José C.. |
| Four different detergents, ASB 14, SB 3-10, CHAPS and Triton X100, were utilized to determine the optimal detergent for the solubilization of membrane proteins from the phytopathogenic bacterium Xylella fastidiosa. These proteins were differentially solubilized in distinct buffers containing the detergent and subjected to bidimensional electrophoresis within the non-linear pH range of 3-10. The detergents ASB 14 and SB 3-10 were the most effective revealing 221 and 157 spots, respectively. CHAPS and Triton X100 were less effective and revealed only 72 and 43 spots, respectively. MALDI-TOF tryptic peptide mass fingerprinting of 18 excised proteins from the ASB 14 treatment revealed that 83% were membrane proteins and that the theoretical efficiency of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: X. fastidiosa; 2-DE; Extraction; Proteome; Solubilization. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200018 |
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| Ferrari,Fernanda; Ludwig,Thelma Alvim Veiga. |
| Realizou-se o levantamento florístico das Coscinodiscophyceae, Fragilariophyceae e Bacillariophyceae (Achnanthales) dos rios Ivaí, São João e dos Patos, pertencentes à bacia hidrográfica do rio Ivaí, município de Prudentópolis, Paraná. Quarenta e uma amostras foram coletadas em março, junho e julho/2002 e janeiro/2003, e analisadas. As coletas fitoplanctônicas foram feitas através de arrasto superficial com rede de plâncton (25 µm) e as perifíticas através da coleta de porções submersas de macrófitas aquáticas, rochas, cascalho, sedimento ou substrato arenoso. Foram identificados, nove táxons pertencentes à classe Coscinodiscophyceae, oito à classe Fragilariophyceae e quinze à ordem Achnanthales (Bacillariophyceae). Thalassiosira weissflogii (Grunow)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diatomáceas; Algas; Ecossistemas lóticos; Taxonomia; Bacillariophyta. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062007000200016 |
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| Pereira Alves,Angela Maria; de Paula,Anderson Luiz; Moreira,Cassio Rafael; Ferrari,Fernanda; Coelho Leal,Jean Paulo; Hatoum,Ualid Saleh; Vieira Silva,Helaine Maruska; Belato Alves,Eder Paulo; de Miranda Neto,Marcilio Hubner; Galvanini,Paulo Alexandre. |
| The effects of quercetin supplementation in NADH-diaphorase positive (NADH-d) neurons of streptozotocin-induced diabetic rats was carried in this study. Fifteen male rats were divided into three groups: normoglycemic (N), diabetic (D) and diabetic supplemented with quercetin (DQ). Whole mount preparations of the muscular layer of the ileum underwent NADH-d histochemistry for evidencing the NADH-d neuronal subpopulation. Quantitative analyzes were performed on 30 random fields, and morphometric analyzes in 100 neuronal bodies and nuclei per animal. The supplementation promoted a 44 % reduction in the neuronal density in D group when compared to N group (p <0.001); a 24.5 % reduction was observed in the DQ group when compared to N (p <0.01). Animals in... |
| Tipo: Journal article |
Palavras-chave: Diabetes mellitus; Enteric neurons; Quercetin; NADH diaphorase. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000100039 |
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